Cloning, sequencing, and expression of the gene encoding Clostridium paraputrificum chitinase ChiB and analysis of the functions of novel cadherin-like domains and a chitin-binding domain.

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Molecular cloning of Clostridium septicum vaccine strain alpha toxin gene in E. coli

Clostridium septicum a Gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. C. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. The aim of the present study was to molecular cloning and sequencing of C. septicum vaccine strain alpha toxin gene. Genomic DNA was extracted using standard phenol and chlorofor...

Overexpression of chimeric chitinase42 enhanced antifungal activity of Trichoderma harzianum against Fusarium graminearum

Evidence for the role of chitinases in biocontrol by Trichoderma species has been well documented.Chit42 lacks a chitin–binding domain (ChBD) which is involved in its binding activity to insoluble chitin. The objective of the present study was to enhance antifungal activity of T. harzianum by overexpression of wild type and hybrid forms of Chit42 containing chitin binding domain. To produce chi...

Cloning and Expression of Two New Recombinant Antimicrobial Dermaseptin B1 Peptides in Tobacco to Control the Growth of Human Bacterial Pathogens

Background and purpose: Rapid emergence of traditional antibiotic-resistant pathogens is one of the most important global challenges in medical sciences. To this end, substitution of current antibiotics with strong antimicrobial peptides could be of great benefit. Materials and methods: In this study, the DNA sequence encoding dermaseptin B1 (DrsB1) antimicrobial peptide derived from Phyllomed...

Cloning and Characterization of cbhII Gene fromTrichoderma parceramosum and Its Expressionin Pichia pastoris

The genomic and cDNA clones encoding cellobiohydrolase II (CBHII) have been isolated and sequenced from a native Iranian isolate of Trichoderma parceramosum, a high cellulolytic enzymes producer isolate. This represents the first report of cbhII gene from this organism. Comparison of genomic and cDNA sequences indicates this gene contains three short introns and also an open reading frame codin...